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The next Helmholtz Hacky Hour will take place on Wednesday, December 9, 2020 from 2PM to 3PM! Topic: Sustainable Programming! more...
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|### External annotation with Diamond and MEGAN6|
|To finalize our analysis process, the annotation is performed externally. This allows for switching to a more powerfull machine as Diamond needs a lot of computational power to generate an NCBI-database based mapping file per sample.|
|In case no suitable computational facilities are availble, consider using computationally less demanding tools such as [Prokka](https://github.com/tseemann/prokka) for annotation.|
|The first step for annotation is to generate an `nr.dmnd` file based on `nr.gz`, downloaded from NCBI, with Diamond. This step must be performed only at the first time:|