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Commit 7e3265e5 authored by Florian Centler's avatar Florian Centler

Update README.md

parent c76eb73c
......@@ -160,6 +160,7 @@ Inside the `$path/` folder, the following sub-folders will be created:
### External annotation with Diamond and MEGAN6
To finalize our analysis process, the annotation is performed externally. This allows for switching to a more powerfull machine as Diamond needs a lot of computational power to generate an NCBI-database based mapping file per sample.
In case no suitable computational facilities are availble, consider using computationally less demanding tools such as [Prokka](https://github.com/tseemann/prokka) for annotation.
The first step for annotation is to generate an `nr.dmnd` file based on `nr.gz`, downloaded from NCBI, with Diamond. This step must be performed only at the first time:
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